In a spectrophotometric assay obeying Beer's law, the absorbance of a sample doubles. What does this indicate about the concentration?
1The concentration is doubled✓ 정답
2The concentration is halved
3The concentration is unchanged
4The relationship cannot be determined
해설
Under the Beer-Lambert law absorbance is directly proportional to concentration, so doubling the absorbance means the concentration has doubled. Remember that this relationship holds only within the linear range and breaks down at high concentrations.