A patient sample shows all panel cells reactive, a reactive autologous control, and a direct antiglobulin test…

Question

A patient sample shows all panel cells reactive, a reactive autologous control, and a direct antiglobulin test positive with both anti-IgG and anti-C3. Which technique is most useful for detecting an underlying alloantibody?

Accepted Answer

보기 3: Adsorption of the patient serum

When an autoantibody reacts with every panel cell, the underlying alloantibody is hidden. Adsorption removes the autoantibody so that the remaining serum can be tested and any alloantibody revealed. Elution identifies antibody bound to the red cells and serves a different purpose.

Answer

보기 1: Enzyme treatment of the patient serum

Answer

보기 2: Elution followed by ABO typing of the eluate

Answer

보기 4: Saline replacement

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A patient sample shows all panel cells reactive, a reactive autologous control, and a direct antiglobulin test positive with both anti-IgG and anti-C3. Which technique is most useful for detecting an underlying alloantibody?

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