A patient sample shows all panel cells reactive, a reactive autologous control, and a direct antiglobulin test positive with both anti-IgG and anti-C3. Which technique is most useful for detecting an underlying alloantibody?
1Enzyme treatment of the patient serum
2Elution followed by ABO typing of the eluate
3Adsorption of the patient serum✓ 정답
4Saline replacement
해설
When an autoantibody reacts with every panel cell, the underlying alloantibody is hidden. Adsorption removes the autoantibody so that the remaining serum can be tested and any alloantibody revealed. Elution identifies antibody bound to the red cells and serves a different purpose.