Why must IgG-coated check cells be added to all negative antihuman globulin tests?

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보기 2: To confirm that active antiglobulin reagent was present and was not neutralized

Incomplete washing leaves residual serum globulins that neutralize the antihuman globulin reagent and produce a false negative. Adding IgG-sensitized cells verifies that the reagent was still active: agglutination confirms validity, and no agglutination invalidates the negative result, which must then be repeated.

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보기 1: To confirm that the patient sample truly contained no unexpected antibody

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보기 3: To confirm that the centrifuge speed and timing were set correctly

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보기 4: To detect complement-dependent antibodies that are missed at the antiglobulin phase

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Why must IgG-coated check cells be added to all negative antihuman globulin tests?

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