A patient with marked hyperproteinemia shows unexpected weak reactions with both reagent reverse-grouping cells. Microscopy suggests rouleaux. Which technique best tests whether the reactions are caused by excess plasma protein?
1Report the patient as group AB immediately without further workup, assuming the weak reactions are true and the discrepancy is due to a technical error
2Add more patient plasma to the test without changing the cell ratio, then recheck for rouleaux under the microscope
3Replace the patient's plasma with saline after centrifugation and repeat the reverse-grouping reactions✓ 정답
4Incubate the test at 56°C for one hour to inactivate cold-reactive antibodies that may be causing the weak reactions
해설
Saline replacement removes the protein-rich plasma while leaving the red-cell aggregates in the tube. Dispersal of rouleaux after replacement supports pseudoagglutination rather than a true antigen-antibody reaction.